Symposium Program Single Molecule Fret Reveals the Pore Size and Opening Mechanism for Mscl Emergence of Rapid Evolution from Demographic Stochas- Ticity
نویسنده
چکیده
The mechanosensitive channel of large conductance (MscL) serves as a model system for mechanosensitive ion channels gated by the bilayer mechanism. Here we report our measurements of the distance changes of multiple residues of MscL using smFRET. These measurements were then used to develop a molecular dynamics model for the open structure of the protein. We find that its open pore size is 2.8 nm in diameter and that the channel opens via the helix-tilt model.
منابع مشابه
Single molecule FRET reveals pore size and opening mechanism of a mechano-sensitive ion channel
The mechanosensitive channel of large conductance, which serves as a model system for mechanosensitive channels, has previously been crystallized in the closed form, but not in the open form. Ensemble measurements and electrophysiological sieving experiments show that the open-diameter of the channel pore is >25 Å, but the exact size and whether the conformational change follows a helix-tilt or...
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http://dx.doi.org/10.7554/elife.01834 Please address questions to [email protected]. Details on how to cite eLife articles in news stories and our media policy are available at http://www.elifesciences.org/news/for-thepress. Articles published in eLife may be read on the journal site at http://elife.elifesciences.org. To be notified of new content at eLife, sign up at http://elife.elifesc...
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تاریخ انتشار 2013